Summary
Type: TLC
Application #: 402270
Catalog#: 1615026 - Silica G TLC Plates, glass backed, 250um, 20x20cm 25/pk
Detection: The plate is dipped into a mixture of 44 ml acetone, 1 ml aniline, 1 g diphenylamine, and 5 ml phosphoric acid (85%) and slightly moved to and fro for 90 sec. Then it is dried again and heated to 150
Conditions: Eluents: Butanol / ethanol / water / acetic acid 5:3:2:0.5
Procedure: The plate is developed until 50 % of the height, dried, developed again until 2/3 of the height, and finally dried.
Authors: Unger C
Source: Faculty of Agricultural Sciences University of Ros